Integrin-Associated Complexes Form Hierarchically with Variable Stoichiometry in Nascent Adhesions

A complex network of putative molecular interactions underlies the architecture and function of cell-matrix adhesions. Most of these interactions are implicated from coimmunoprecipitation studies using expressed components, but few have been demonstrated or characterized functionally in living cells. We introduce fluorescence fluctuation methods to determine, at high spatial and temporal resolution, “when” and “where” molecular complexes form and their stoichiometry in nascent adhesions (NAs). We focus on integrin-associated molecules implicated in integrin activation and in the integrin-actin linkage in NAs and show that these molecules form integrin-containing complexes hierarchically within the adhesion itself. Integrin and kindlin reside in a molecular complex as soon as adhesions are visible; talin, although also present early, associates with the integrin-kindlin complex only after NAs have formed and in response to myosin II activity. Furthermore, talin and vinculin association precedes the formation of the integrin-talin complex. Finally, α-actinin enters NAs periodically and in clusters that transiently associate with integrins. The absolute number and stoichiometry of these molecules varies among the molecules studied and changes as adhesions mature. These observations suggest a working model for NA assembly whereby transient α-actinin-integrin complexes help nucleate NAs within the lamellipodium. Subsequently, integrin complexes containing kindlin, but not talin, emerge. Once NAs have formed, myosin II activity promotes talin association with the integrin-kindlin complex in a stoichiometry consistent with each talin molecule linking two integrin-kindlin complexes. •α-actinin clusters transiently associate with integrins as adhesions form•Integrin associates with kindlin, but not talin, during adhesion assembly•Integrin-talin complex forms in response to myosin II activity as adhesions mature•The ratio of molecules differs in nascent versus focal adhesions Bachir et al. use high-resolution fluorescence correlation microscopy to determine when integrin-associated molecular complexes form and their stoichiometry in nascent adhesions. The observations reveal changing associations within the adhesion and a variable stoichiometry that also changes as the adhesion forms and matures.