An intact signal peptide on dengue virus E protein enhances immunogenicity for CD8+ T cells and antibody when expressed from modified vaccinia Ankara

Highlights • MVA vectored dengue vaccines were explored for their CD8+ T cells immunogenicity. • We identified two H-2b -restricted DENV-3 E protein-specific CD8+ T cell epitopes. • rMVA vaccines expressing E protein elicited CD8+ T cell and antibody responses. • Vaccines encoding the E protein with...

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Veröffentlicht in:	Vaccine 2014-05, Vol.32 (25), p.2972-2979
Hauptverfasser:	Quinan, Bárbara R, Flesch, Inge E.A, Pinho, Tânia M.G, Coelho, Fabiana M, Tscharke, David C, da Fonseca, Flávio G
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies, Viral - blood Antibody Formation Applied microbiology Biological and medical sciences CD8-Positive T-Lymphocytes - immunology Cloning Cytokines Dengue - prevention & control Dengue fever Dengue Vaccines - immunology Dengue Virus Epitopes, T-Lymphocyte - immunology Female Fundamental and applied biological sciences. Psychology Gene expression Genetic Vectors Immune response Immunogenicity Infections Lymphocytes Mice, Inbred BALB C Mice, Inbred C57BL Microbiology Miscellaneous Mutagenesis Peptides Plasmids Protein Sorting Signals Proteins Public health Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccinia virus Vaccinia virus - genetics Vaccinia virus - immunology Vector-borne diseases Viral Envelope Proteins - immunology Virology Viruses Visual aids
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Zusammenfassung:	Highlights • MVA vectored dengue vaccines were explored for their CD8+ T cells immunogenicity. • We identified two H-2b -restricted DENV-3 E protein-specific CD8+ T cell epitopes. • rMVA vaccines expressing E protein elicited CD8+ T cell and antibody responses. • Vaccines encoding the E protein with signal sequences had superior immunogenicity.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2014.03.093