Effect of Heat Shock Protein 90 (Hsp90) on Migration and Invasion of Human Cancer Cells in Vitro
We studied the effect of purifi ed native heat shock protein 90 (Hsp90) from bovine and mouse brain on migration and invasion of human glioblastoma (A-172) and fi brosarcoma (HT1080) cells. Hsp90 in concentrations of 0.01-0.10 mg/ml stimulated migration and invasion of tumor cells in vitro by 20-32%...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2014-08, Vol.157 (4), p.476-478 |
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| creator | Snigireva, A. V. Vrublevskaya, V. V. Skarga, Yu. Yu Evdokimovskaya, Yu. V. Morenkov, O. S. |
| description | We studied the effect of purifi ed native heat shock protein 90 (Hsp90) from bovine and mouse brain on migration and invasion of human glioblastoma (A-172) and fi brosarcoma (HT1080) cells. Hsp90 in concentrations of 0.01-0.10 mg/ml stimulated migration and invasion of tumor cells
in vitro
by 20-32% (
p |
| doi_str_mv | 10.1007/s10517-014-2595-9 |
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in vitro
by 20-32% (
p
<0.05). Polyclonal antibodies to Hsp90 blocked the Hsp90-dependent stimulation of cell invasion, which indicates specifi city of the stimulating effect of extracellular Hsp90 on tumor cell invasion. Hence, extracellular Hsp90 can be considered as a promising molecular target, because its inhibition can suppress invasion and metastasizing of tumor cells.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-014-2595-9</identifier><identifier>PMID: 25110087</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Antibodies - pharmacology ; Biomedical and Life Sciences ; Biomedicine ; Brain Chemistry ; Cancer ; Cattle ; Cell Biology ; Cell Line, Tumor ; Cell Movement - drug effects ; Diffusion Chambers, Culture ; Extracellular Matrix Proteins - pharmacology ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Heat shock proteins ; HSP70 Heat-Shock Proteins - antagonists & inhibitors ; HSP70 Heat-Shock Proteins - isolation & purification ; HSP70 Heat-Shock Proteins - pharmacology ; HSP90 Heat-Shock Proteins - antagonists & inhibitors ; HSP90 Heat-Shock Proteins - isolation & purification ; HSP90 Heat-Shock Proteins - pharmacology ; Internal Medicine ; Laboratory Medicine ; Mice ; Neuroglia - cytology ; Neuroglia - drug effects ; NIH 3T3 Cells ; Pathology ; Sarcoma</subject><ispartof>Bulletin of experimental biology and medicine, 2014-08, Vol.157 (4), p.476-478</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-b2e26d6564ac5b5b94dba9eb2a7e972b18a844e4fc374966d9a3e188c97774603</citedby><cites>FETCH-LOGICAL-c540t-b2e26d6564ac5b5b94dba9eb2a7e972b18a844e4fc374966d9a3e188c97774603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-014-2595-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-014-2595-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25110087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Snigireva, A. V.</creatorcontrib><creatorcontrib>Vrublevskaya, V. V.</creatorcontrib><creatorcontrib>Skarga, Yu. Yu</creatorcontrib><creatorcontrib>Evdokimovskaya, Yu. V.</creatorcontrib><creatorcontrib>Morenkov, O. S.</creatorcontrib><title>Effect of Heat Shock Protein 90 (Hsp90) on Migration and Invasion of Human Cancer Cells in Vitro</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We studied the effect of purifi ed native heat shock protein 90 (Hsp90) from bovine and mouse brain on migration and invasion of human glioblastoma (A-172) and fi brosarcoma (HT1080) cells. Hsp90 in concentrations of 0.01-0.10 mg/ml stimulated migration and invasion of tumor cells
in vitro
by 20-32% (
p
<0.05). Polyclonal antibodies to Hsp90 blocked the Hsp90-dependent stimulation of cell invasion, which indicates specifi city of the stimulating effect of extracellular Hsp90 on tumor cell invasion. Hence, extracellular Hsp90 can be considered as a promising molecular target, because its inhibition can suppress invasion and metastasizing of tumor cells.</description><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain Chemistry</subject><subject>Cancer</subject><subject>Cattle</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Diffusion Chambers, Culture</subject><subject>Extracellular Matrix Proteins - pharmacology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Heat shock proteins</subject><subject>HSP70 Heat-Shock Proteins - antagonists & inhibitors</subject><subject>HSP70 Heat-Shock Proteins - isolation & purification</subject><subject>HSP70 Heat-Shock Proteins - pharmacology</subject><subject>HSP90 Heat-Shock Proteins - antagonists & inhibitors</subject><subject>HSP90 Heat-Shock Proteins - isolation & purification</subject><subject>HSP90 Heat-Shock Proteins - pharmacology</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Mice</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - drug effects</subject><subject>NIH 3T3 Cells</subject><subject>Pathology</subject><subject>Sarcoma</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kV1rFDEUhoModlv9Ad5IQJB6MTXJ5POyLK1bqCj4cRszmTO7U2eSNZkR_Pdm2KqtKLlITvK8L-fkRegZJWeUEPU6UyKoqgjlFRNGVOYBWlGh6kozRh-iFSlQxbXWR-g455ulJJI-RkdM0GKg1Qp9ueg68BOOHd6Am_CHXfRf8fsUJ-gDNgSfbvLekFc4Bvy23yY39eXkQouvwneXl2KRzqMLeO2Ch4TXMAwZF_XnfkrxCXrUuSHD09v9BH26vPi43lTX795crc-vKy84maqGAZOtFJI7LxrRGN42zkDDnAKjWEO105wD73ytuJGyNa4GqrU3SikuSX2CTg---xS_zZAnO_bZl1ZcgDhnS4XgXBpiZEFf_IXexDmF0p2lStWMU6nrP9TWDWD70MUpOb-Y2vNay_KZkppCnf2DKquFsfcxQNeX-3uCl3cEO3DDtMtxmJd_zfdBegB9ijkn6Ow-9aNLPywldonfHuK3JX67xG8XzfPbyeZmhPa34lfeBWAHIJensIV0Z_T_uv4Ev820Tw</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Snigireva, A. 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V.</au><au>Vrublevskaya, V. V.</au><au>Skarga, Yu. Yu</au><au>Evdokimovskaya, Yu. V.</au><au>Morenkov, O. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Heat Shock Protein 90 (Hsp90) on Migration and Invasion of Human Cancer Cells in Vitro</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>157</volume><issue>4</issue><spage>476</spage><epage>478</epage><pages>476-478</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><coden>BEXBAN</coden><abstract>We studied the effect of purifi ed native heat shock protein 90 (Hsp90) from bovine and mouse brain on migration and invasion of human glioblastoma (A-172) and fi brosarcoma (HT1080) cells. Hsp90 in concentrations of 0.01-0.10 mg/ml stimulated migration and invasion of tumor cells
in vitro
by 20-32% (
p
<0.05). Polyclonal antibodies to Hsp90 blocked the Hsp90-dependent stimulation of cell invasion, which indicates specifi city of the stimulating effect of extracellular Hsp90 on tumor cell invasion. Hence, extracellular Hsp90 can be considered as a promising molecular target, because its inhibition can suppress invasion and metastasizing of tumor cells.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>25110087</pmid><doi>10.1007/s10517-014-2595-9</doi><tpages>3</tpages></addata></record> |
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| ispartof | Bulletin of experimental biology and medicine, 2014-08, Vol.157 (4), p.476-478 |
| issn | 0007-4888 1573-8221 |
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| subjects | Animals Antibodies - pharmacology Biomedical and Life Sciences Biomedicine Brain Chemistry Cancer Cattle Cell Biology Cell Line, Tumor Cell Movement - drug effects Diffusion Chambers, Culture Extracellular Matrix Proteins - pharmacology Fibroblasts - cytology Fibroblasts - drug effects Heat shock proteins HSP70 Heat-Shock Proteins - antagonists & inhibitors HSP70 Heat-Shock Proteins - isolation & purification HSP70 Heat-Shock Proteins - pharmacology HSP90 Heat-Shock Proteins - antagonists & inhibitors HSP90 Heat-Shock Proteins - isolation & purification HSP90 Heat-Shock Proteins - pharmacology Internal Medicine Laboratory Medicine Mice Neuroglia - cytology Neuroglia - drug effects NIH 3T3 Cells Pathology Sarcoma |
| title | Effect of Heat Shock Protein 90 (Hsp90) on Migration and Invasion of Human Cancer Cells in Vitro |
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