Rapid assay of spermidine synthase activity by high-performance liquid chromatography

In this paper, the authors describe a rapid and economic high-performance liquid chromatographic (HPLC) method for the separation and quantitation of spermidine formed upon incubation of putrescine with a crude extract of mouse brain. This method thus permits the rapid measurement of spermidine synt...

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Veröffentlicht in:	Journal of Chromatography A 1981-01, Vol.226 (1), p.208-212
Hauptverfasser:	Porta, R., Esposito, C., Sellinger, O.Z.
Format:	Artikel
Sprache:	eng
Schlagworte:	aminopropyltransferase Animals assays brain Brain - enzymology Chromatography, High Pressure Liquid - methods In Vitro Techniques Kinetics Male Mice Putrescine - metabolism Spermidine Synthase - analysis Spermidine Synthase - metabolism Transferases - metabolism
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Beschreibung
Zusammenfassung:	In this paper, the authors describe a rapid and economic high-performance liquid chromatographic (HPLC) method for the separation and quantitation of spermidine formed upon incubation of putrescine with a crude extract of mouse brain. This method thus permits the rapid measurement of spermidine synthase (EC 2.5.1.16) activity.
ISSN:	0378-4347 0021-9673
DOI:	10.1016/S0378-4347(00)84223-1