Detection of polymorphism in exon 26 of apoB gene in Khazak breed chickens

The aim of the present study was to detect the polymorphism in exon 26 of apoB gene in Khazak breed Chickens. Apolipoprotein B (apoB) is the primary apolipoprotein of low density lipoproteins, and is responsible for carrying cholesterol to tissues. The apoB gene is located on chromosome 3 and consists of thirty exons and twentynine introns. in this study, sixty sample selected of laying chickens native khazak breed to the region of Sistan and Baluchestan and DNA genomic was extracted from follicle end feather and genotyping by the PCR-RFLP method. The PCR product was digested with restriction enzyme Acyl and then separated electrophoresis using 2% agars gel. A mutation was identified on exon 26 in chromosome 3. AA, AB and BB Genotypes were detected in this population, respectively with frequency of 16.7, 28.3 and 55 percent. The frequency of an allele and B allele was estimated, respectively 0.3083 and 0.6917 percent. The B allele was more frequent than B allele and BB genotype was more frequent than other genotypes in this population. Chi square (X super(2)) test and G test showed Hardy-Weinberg equilibrium in the population and this polymorphism was approved using sequencing method with ABI sequencing system. Thus, according to the results observed and the important role of this gene in egg production, this gene appears to be an important candidate gene in native chickens used in breeding program.