Raman ratios on the repair of grafted surgical bone defects irradiated or not with laser (λ780nm) or LED (λ850nm)

[Display omitted] •Repair of large bone defects may be timing consuming and improper.•We studied the effects of association of biomaterial and IR Laser or IR LED light.•Raman metrics indicate of a positive effect of the association on the repair.•The mineralization was intense in grafted defects at early remodeling time.•Collagen level decreased with time and bone mineralization. This work aimed to assess biochemical changes associated to mineralization and remodeling of bone defects filled with Hydroxyapatite+Beta-Beta-tricalcium phosphate irradiated or not with 2 light sources. Ratios of intensities, band position and bandwidth of selected Raman peaks of collagen and apatites were used. Sixty male Wistar rats were divided into 6 groups subdivided into 2 subgroups (15th and 30thdays). A standard surgical defect was created on one femur of each animal. In 3 groups the defects were filled with blood clot (Clot, Clot+Laser and Clot+LED groups) and in the remaining 3 groups the defects were filled with biomaterial (Biomaterial, Biomaterial+Laser and Biomaterial+LED groups). When indicated, the defects were irradiated with either Laser (λ780nm, 70mW, Φ∼0.4cm2) or LED (λ850±10nm, 150mW, Φ∼0.5cm2), 20J/cm2 each session, at 48h intervals/2weeks (140J/cm2 treatment). Following sacrifice, bone fragments were analyzed by Raman spectroscopy. Statistical analysis (ANOVA General Linear Model, p