Terbium promotes adhesion and osteogenic differentiation of mesenchymal stem cells via activation of the Smad-dependent TGF-β/BMP signaling pathway
With its special physical and chemical properties, terbium has been widely used, which has inevitably increased the chance of human exposure to terbium-based compounds. It was reported that terbium mainly deposited in bone after introduction into the human body. Although some studies revealed the ef...
Gespeichert in:
Veröffentlicht in: | Journal of biological inorganic chemistry 2014-08, Vol.19 (6), p.879-891 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | With its special physical and chemical properties, terbium has been widely used, which has inevitably increased the chance of human exposure to terbium-based compounds. It was reported that terbium mainly deposited in bone after introduction into the human body. Although some studies revealed the effects of terbium on bone cell lines, there have been few reports about the potential effect of terbium on adhesion and differentiation of mesenchymal stem cells (MSCs). In this study, we investigated the effects of terbium on the adhesion and osteogenic and adipogenic differentiation of MSCs and the associated molecular mechanisms. Our data reveal that terbium promoted the osteogenic differentiation in a time-dependent manner and conversely inhibited the adipogenic differentiation of MSCs. Meanwhile, the cell–cell or cell–matrix interaction was enhanced by activating adherent-related key factors, which were evaluated by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Real-time RT-PCR and Western blot analysis were also performed to further detect osteogenic and adipogenic biomarkers of MSCs. The regulation of terbium on differentiation of MSCs led to the interaction between the transforming growth factor β/bone morphogenetic protein and peroxisome-proliferator-activated receptor γ (PPARγ) signaling pathways, resulting in upregulation of the osteogenic master transcription factors, such as Runt-related transcription factor 2, bone morphogenetic protein 2, collagen I, alkaline phosphatase, and osteocalcin, and downregulation of the adipogenic master transcription factors, such as PPARγ
2
. The results provide novel evidence to elucidate the mechanisms of bone metabolism by terbium and may be helpful for more rational application of terbium-based compounds in the future.
Graphical abstract
The effects of terbium on the osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms were investigated. The results suggest that terbium promotes the osteogenic differentiation of MSCs via the transforming growth factor β (
TGFβ
)/bone morphogenetic protein (
BMP
) signaling pathway.
ALP
alkaline phosphatase,
BSP
bone sialoprotein,
C/EBP
CCAAT/enhancer binding protein,
Col I
collagen I,
ERα
estrogen receptor α,
GDF
growth differentiation factor,
OCN
osteocalcin,
PPARγ
peroxisome-proliferator-activated receptor γ,
Runx2
Runt-related transcription factor 2 |
---|---|
ISSN: | 0949-8257 1432-1327 |
DOI: | 10.1007/s00775-014-1119-4 |