Mechanism of repression of 11β-hydroxysteroid dehydrogenase type 1 by growth hormone in 3T3-L1 adipocytes

11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is an NADPH-dependent reductase that converts cortisone to cortisol in adipose tissue. We previously reported that GH and IGF-I decrease 11β-HSD1 activity and mRNA levels in adipocytes. Hexose-6-phosphate dehydrogenase (H6PDH) is involved in the pro...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Endocrine Journal 2014, Vol.61(7), pp.675-682
Hauptverfasser:	Muraoka, Toko, Hizuka, Naomi, Fukuda, Izumi, Ishikawa, Yukiko, Ichihara, Atsuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	11-beta-Hydroxysteroid Dehydrogenase Type 1 - antagonists & inhibitors 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) 3T3-L1 Cells Adipocytes Adipocytes, White - drug effects Adipocytes, White - enzymology Adipocytes, White - metabolism Animals Carbohydrate Dehydrogenases - antagonists & inhibitors Carbohydrate Dehydrogenases - genetics Carbohydrate Dehydrogenases - metabolism Enzyme Repression - drug effects Growth hormone (GH) Growth Hormone - metabolism Hexose-6-phosphate dehydrogenase (H6PDH) Imidazoles - pharmacology Insulin - metabolism Insulin Resistance Insulin-Like Growth Factor I - antagonists & inhibitors Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - metabolism Insulin-like growth factor-I (IGF-I) Mice Phosphorylation - drug effects Phthalazines - pharmacology Protein Kinase Inhibitors - pharmacology Protein Processing, Post-Translational - drug effects Pyrazines - pharmacology Pyridines - pharmacology Receptor, IGF Type 1 - antagonists & inhibitors Receptor, IGF Type 1 - genetics Receptor, IGF Type 1 - metabolism RNA, Messenger - metabolism Signal Transduction - drug effects
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is an NADPH-dependent reductase that converts cortisone to cortisol in adipose tissue. We previously reported that GH and IGF-I decrease 11β-HSD1 activity and mRNA levels in adipocytes. Hexose-6-phosphate dehydrogenase (H6PDH) is involved in the production of NADPH, which is a coenzyme for 11β-HSD1. The aim of the present study was to clarify further the mechanism of repression of 11β-HSD1 activity by GH using linsitinib, an IGF-I receptor inhibitor. The suppression of 11β-HSD1 mRNA by IGF-I was attenuated in the presence of 1 μM linsitinib (17.2% vs. 53.3% of basal level, P
ISSN:	0918-8959 1348-4540
DOI:	10.1507/endocrj.EJ13-0528