A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

Highlights • We developed a novel identification method for vaginal secretions. • This method is based on relative quantification of vaginal Lactobacillus species. • The measured ΔCt values were significantly low in vaginal secretions. • This ΔCt method is simple and sensitive. • The ΔCt value can b...

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Veröffentlicht in:Forensic science international : genetics 2014-09, Vol.12, p.93-99
Hauptverfasser: Doi, Masanori, Gamo, Shinsuke, Okiura, Tatsuyuki, Nishimukai, Hiroaki, Asano, Migiwa
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
DNA Primers
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Female
Humans
Lactobacillus - genetics
Pathology
Real-Time Polymerase Chain Reaction
RNA, Ribosomal, 16S - genetics
Vagina - microbiology
Vagina - secretion
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Beschreibung
Zusammenfassung:Highlights • We developed a novel identification method for vaginal secretions. • This method is based on relative quantification of vaginal Lactobacillus species. • The measured ΔCt values were significantly low in vaginal secretions. • This ΔCt method is simple and sensitive. • The ΔCt value can be used as an indicator for the presence of vaginal secretions.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2014.05.005