Glucose Production Using Immobilized Mycelial-Associated beta -Glucosidase of Trichoderma E58

The immobilization of the mycelial-associated beta -glucosidase of Trichoderma E-58 ha been carried out by encapsulating, in calcium alginate beads, the fungal mycelium obtained during liquid culture. The activity of this immobilized beta -glucosidase was found to vary with culture age and to be more thermally stable than the extracellular beta -glucosidase produced by this organism. The activity of the immobilized enzyme was successfully demonstrated in both static and shake-flask batch reaction mixtures at 50 degree C using both cellobiose and salicin as substrates.