Prostaglandin Transporting Protein-mediated Prostaglandin E2 Transport in Lipopolysaccharide-inflamed Rat Dental Pulp

Abstract Introduction The prostaglandin transporter (Pgt) and multidrug resistance-associated protein (Mrp) 4 are membrane transport proteins that play crucial roles in the transmembrane uptake and/or efflux of prostaglandins (PGs). This study attempted to analyze the protein expression of Pgt and M...

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Veröffentlicht in:Journal of endodontics 2014-08, Vol.40 (8), p.1112-1117
Hauptverfasser: Ohkura, Naoto, DDS, Shigetani, Yoshimi, DDS, PhD, Yoshiba, Nagako, DDS, PhD, Yoshiba, Kunihiko, DDS, PhD, Okiji, Takashi, DDS, PhD
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Sprache:eng
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Zusammenfassung:Abstract Introduction The prostaglandin transporter (Pgt) and multidrug resistance-associated protein (Mrp) 4 are membrane transport proteins that play crucial roles in the transmembrane uptake and/or efflux of prostaglandins (PGs). This study attempted to analyze the protein expression of Pgt and Mrp4 and their involvement in PGE2 efflux transport in lipopolysaccharide (LPS)-inflamed rat incisor pulp tissue. Methods Pulpitis was induced in the upper incisors of Wistar rats by treating them with LPS for 24 hours. The protein expression levels of Pgt, Mrp4, and microsomal PGE synthase (mPGES) were analyzed with immunofluorescent staining. The amount of PGE2 released from the inflamed pulp tissue in the presence or absence of dipyridamole (an Mrp4 inhibitor) was assessed by using an enzyme-linked immunosorbent assay. Results Double immunofluorescence staining revealed that the Pgt, Mrp4, and mPGES immunoreactivity co-localized in CD31-expressing endothelial cells. Moreover, the Mrp4 inhibitor caused a significant decrease in the amount of PGE2 released from the LPS-inflamed pulp ( P  
ISSN:0099-2399
1878-3554
DOI:10.1016/j.joen.2013.12.024