Quantification of five plasticizers used in PVC tubing through high performance liquid chromatographic-UV detection

•The development of a rapid and simple technique for 4 DEHP-substitute plasticizers.•The ability to measure the DINCH by HPLC–UV with other plasticizers.•The validation of quantification by the total error (accuracy profile) approach.•The ability to measure these DEHP-substitute plasticizers after extraction from PVC tubing. Searching for alternatives to di-(2-ethylhexyl)-phthalate, a plasticizer that has been widely used in the manufacturing of PVC medical devices, has become a major challenge since a European regulation underlined some clinical risks. The aim of this study is to develop an HPLC–UV method to quantify the currently used alternative plasticizers to DEHP. Five plasticizers, acetyl tributyl citrate, di-(2-ethylhexyl)-phthalate, di-(ethylhexyl)-terephthalate, di-isononyl-1,2-cyclohexane-dicarboxylate, and trioctyl trimellitate, were separated on a C8 stationary phase (2.6μm, 100mm×4.6mm) under gradient elution in 13min. They were detected at 221nm leading to a quantification threshold from 0.3 to 750μg/mL as a function of the plasticizer. Within-day and between-day precisions were inferior to 0.9% and 18%, respectively. The assays were validated according to the accuracy profile method. Plasticizers were extracted from PVC-tubing by dissolving PVC in THF then precipitating it in methanol with a yield of over 90% for each plasticizer. This assay could feasibly be used to quantify plasticizers in PVC medical devices.