A molecular and structural analysis of the V sub(H) and V sub(K) regions of monoclonal antibodies bearing the A48 regulatory idiotype

The results presented in this paper explore the molecular basis for expression of the A48 regulatory Id (RI). A48 RI super(+) mAb derived from idiotypically manipulated mice molecularly resembled the A48 and UPC 10 prototypes of this system by utilizing a V sub(H)X24-V sub(K)10 combination. Id expression by these antibodies was not restricted by a particular D region sequence, J sub(H), or J sub(K) segment, but quantitative differences in Id expression were associated with utilization of different members of the V sub(K)10 germline gene families.