Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

SUMMARY This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two‐dimensional system (control) or a three‐dimensional encapsulated system in 0.25%, 0.5%, or 1%...

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Veröffentlicht in:	Molecular reproduction and development 2014-07, Vol.81 (7), p.636-645
Hauptverfasser:	Brito, I.R., Silva, C.M.G., Duarte, A.B.G., Lima, I.M.T., Rodrigues, G.Q., Rossetto, R., Sales, A.D., Lobo, C.H., Bernuci, M.P., Rosa-e-Silva, A.C.J.S., Campello, C.C., Xu, M., Figueiredo, J.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Hydroxysteroid Dehydrogenases - analysis 3-Hydroxysteroid Dehydrogenases - genetics 3-Hydroxysteroid Dehydrogenases - metabolism Alginates - chemistry Alginates - pharmacology Animals Aromatase - analysis Aromatase - genetics Aromatase - metabolism Cell Culture Techniques Female Goats Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology Oocytes - drug effects Oocytes - growth & development Ovarian Follicle - drug effects Ovarian Follicle - growth & development
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Zusammenfassung:	SUMMARY This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two‐dimensional system (control) or a three‐dimensional encapsulated system in 0.25%, 0.5%, or 1% alginate (ALG 0.25, ALG 0.5, and ALG 1, respectively). A higher percentage of morphologically normal follicles developed in ALG 0.5 and ALG 1 than in ALG 0.25 or the control (P
ISSN:	1040-452X 1098-2795
DOI:	10.1002/mrd.22330