Rat liver NAD(P)H: Quinone reductase. Regulation of quinone reductase gene expression by planar aromatic compounds and determination of the exon structure of the quinone reductase structural gene
We have determined the effect of beta-naphthoflavone and the azo dye, sudan III, on the level of quinone reductase mRNA in a responsive rat hepatoma cell line. Our data indicate that both of these planar aromatic compounds produce a 4-5-fold elevation in quinone reductase mRNA. The induction of quin...
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Veröffentlicht in: | The Journal of biological chemistry 1989-12, Vol.264 (36), p.21793-21797 |
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Zusammenfassung: | We have determined the effect of beta-naphthoflavone and the azo dye, sudan III, on the level of quinone reductase mRNA in
a responsive rat hepatoma cell line. Our data indicate that both of these planar aromatic compounds produce a 4-5-fold elevation
in quinone reductase mRNA. The induction of quinone reductase mRNA can be blocked by cycloheximide, suggesting a requirement
for ongoing protein synthesis in the induction process. We have determined the exon structure of the quinone reductase structural
gene. The gene is separated into six exons by five introns. A "TATA" box is located 29 base pairs upstream from the transcription
initiation site. A "CCAAT" sequence is found at position -129, and an inverted "GC" box is located at position -78. Quinone
reductase promoter-chlor-amphenicol acetyltransferase fusion genes containing different lengths of the 5'-flanking region
were transfected into rat and human hepatoma cells. Treatment of the transfected cells with beta-naphthoflavone or sudan III
resulted in a 4-5-fold elevation in chloramphenicol acetyltransferase activity. These data suggest the presence of a cis-acting
regulatory element(s) in the 5'-flanking region of the quinone reductase structural gene which regulates inducible expression. |
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ISSN: | 0021-9258 1083-351X |