Characterization and quantification of saponins and flavonoids in sprouts, seed coats and cotyledons of germinated black beans

► Characterization of saponins in black bean seed coats, hilum and cotyledons. ► Quantification of saponins in seed tissues of raw and germinated black beans. ► Sprouts had a higher concentration of saponins compared to cotyledons or seed coats. ► Soyasaponin Af was the most abundant saponin in all black bean tissues. ► Flavonols were mainly concentrated in seed coats. Saponins, flavonols and isoflavones were quantified in sprouts, cotyledons and seed coats of black beans (Phaseolus vulgaris L.) subjected to germination over five days. Sprouts had a higher concentration of saponins compared to cotyledons or seed coats (p