Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface
Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes us...
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| Veröffentlicht in: | Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2014-04, Vol.8 (2), p.164-167 |
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| container_title | Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry |
| container_volume | 8 |
| creator | Presnova, G. V. Rubtsova, M. Yu Presnov, D. E. Grigorenko, V. G. Yaminsky, I. V. Egorov, A. M. |
| description | Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes using a streptavidin conjugate with gold nanoparticles. Effective imaging of DNA duplexes was possible using the conjugate prepared by covalent binding. The detection limit of the model oligonucleotide of 19 bases was 20 pg. |
| doi_str_mv | 10.1134/S1990750814020103 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1990-7508 |
| ispartof | Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry, 2014-04, Vol.8 (2), p.164-167 |
| issn | 1990-7508 1990-7516 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Bioorganic Chemistry Chemistry Chemistry and Materials Science Deoxyribonucleic acid DNA Hybridization Medicinal Chemistry Nanoparticles Scanning electron microscopy |
| title | Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface |
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