Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes us...

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Veröffentlicht in:Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2014-04, Vol.8 (2), p.164-167
Hauptverfasser: Presnova, G. V., Rubtsova, M. Yu, Presnov, D. E., Grigorenko, V. G., Yaminsky, I. V., Egorov, A. M.
Format: Artikel
Sprache:eng
Schlagworte:
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
Hybridization
Medicinal Chemistry
Nanoparticles
Scanning electron microscopy
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Zusammenfassung:Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes using a streptavidin conjugate with gold nanoparticles. Effective imaging of DNA duplexes was possible using the conjugate prepared by covalent binding. The detection limit of the model oligonucleotide of 19 bases was 20 pg.
ISSN:1990-7508
1990-7516
DOI:10.1134/S1990750814020103