Species distribution and antifungal susceptibility of Candida spp. isolated from superficial candidiasis in outpatients in Iran

Candidiasis is the most prevalent fungal infection affecting human and animals all over the world. This study represents the epidemiological aspects of superficial candidiasis in outpatients and in vitro antifungal susceptibility of etiologic Candida species. Clinical samples were taken from 173 patients including skin and nail scrapings (107; 61.8%), vaginal discharge (28; 16.2%), sputum (20; 11.6%), oral swabs (7; 4.0%), bronchoalveolar lavage (6; 3.5%) and 1 specimen (0.6%) of each eye tumor, gastric juice, urine, biopsy and urinary catheter and confirmed as candidiasis by direct microscopy, culture and histopathology. Susceptibility patterns of the isolated Candida species were determined using the disk diffusion and broth microdilution methods. Among 173 Candida isolates, C. albicans (72.3%) was the most prevalent species followed by C. parapsilosis (11.5%). Other identified species were C. glabrata, C. krusei, C. tropicalis, C. guilliermondii, C. intermedia and C. sake. Majority of the Candida isolates were susceptible to fluconazole (95.4%) followed by 5-flucytosine (89.6%), voriconazole (78.6%) itraconazole (48.0%) and ketoconazole (42.8%). Caspofungin was the most potent antifungal drug against C. albicans (MICs; 0.062–1μg/mL), ketoconazole for C. parapsilosis and C. tropicalis (MICs; 0.031–0.25μg/mL) and itraconazole for C. krusei, C. glabrata and C. guilliermondii (MICs; 0.031–1μg/mL). This study reinforces the significance of superficial candidiasis as an important fungal infection with multiple clinical presentations. Our results further indicate that susceptibility testing to commonly used antifungals is crucial in order to select the appropriate therapeutic strategies which minimize complications while improving patients’ life. La candidose est l’infection fongique la plus courante chez les animaux et l’homme partout dans le monde. Cette étude présente les aspects épidémiologiques de la candidose superficielle chez les patients et la sensibilité antifongique in vitro des espèces de Candida isolées. Les prélèvements cliniques ont été effectués sur 173 patients, il s’agit de la peau et des ongles (107 ; 61,8 %), des secrétions vaginales (28 ; 16,2 %), de l’expectoration (20 ; 11,6 %), de prélèvements oraux (7 ; 4,0 %), de lavage broncho-alvéolaire (6 ; 3,5 %) et d’un échantillon (0,6 %) de tumeur de l’œil, de suc gastrique, d’urine, de biopsie et d’un cathéter urinaire. Ils ont été confirmés par l’examen direct, la culture et l’histopathologie