Isolation and Structures of Axistatins 1–3 from the Republic of Palau Marine Sponge Agelas axifera Hentschel

An investigation begun in 1979 directed at the Republic of Palau marine sponge Agelas axifera Hentschel for cancer cell growth inhibitory constituents subsequently led to the isolation of three new pyrimidine diterpenes designated axistatins 1 (1), 2 (2), and 3 (3), together with the previously reported formamides 4, 5, and agelasine F (6). The structures were elucidated by analysis of 2D-NMR spectra and by HRMS. All of the isolated compounds were found to be moderate inhibitors of cancer cell growth. Axistatins 1–3 (1–3), formamide 4, and agelasine F (6) also exhibited antimicrobial activity.