Rapid identification of Vibrio anguillarum by Colony hybridization

Summary A 562 base pair fragment of DNA from a serotype A strain of Vibrio anguillarum was cloned into pUC9 and used as a hybridization probe for the rapid identification of Vibrio anguillarum by colony hybridization. The probe was tested on nine different fish pathogens, 15 Vibrio isolates, 2 organ...

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Veröffentlicht in:	Journal of applied ichthyology 1989-06, Vol.5 (2), p.67-73
Hauptverfasser:	Aoki, Takashi, Hirono, Ikuo, de Castro, Teresa, Kitao, Tadatoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Brackish Freshwater Fundamental and applied biological sciences. Psychology Microbiology Plecoglossus altivelis Teleostei Vibrio anguillarum
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Beschreibung
Zusammenfassung:	Summary A 562 base pair fragment of DNA from a serotype A strain of Vibrio anguillarum was cloned into pUC9 and used as a hybridization probe for the rapid identification of Vibrio anguillarum by colony hybridization. The probe was tested on nine different fish pathogens, 15 Vibrio isolates, 2 organisms closely related to Vibrio, and 9 serotypes of V. anguillarum. The probe hybridized only with the DNA of V. anguillarum serotypes A and H. The sequence of the 562 nucleotides have been determined. This probe allows rapid, reliable, and specific detection of V. anguillarum in freshwater ayu, Plecoglossus altivelis.
ISSN:	0175-8659 1439-0426
DOI:	10.1111/j.1439-0426.1989.tb00475.x