MCF-Specific Murine Monoclonal Antibodies Made Against AKR-247 MCF Virus Recognize a Unique Determinant Associated With the gp70-p15(E) Complex

Hybridomas obtained from (NFS x AKR)F sub(1) mice immunized with syngeneic cells infected with AKR-247 MCF virus produced antibodies specific for only AKR-247 or closely related MCF viruses which share a previously defined MCF antigen (MCFA-3). These monoclonal antibodies recognized a new type of viral antigenic determinant which appeared to be conformational determinant associated with the env) precursor polyprotein (pr80 super(env)) or its disulfide-linked gp70-p15(E) complex (gp80) but not with free gp70 or p15(E) or any other virion or virus-induced protein.