Ultrasensitive and Closed-Tube Colorimetric Loop-Mediated Isothermal Amplification Assay Using Carboxyl-Modified Gold Nanoparticles

Ultrasensitive and Closed-Tube Colorimetric Loop-Mediated Isothermal Amplification Assay Using Carboxyl-Modified Gold Nanoparticles

LAMP ends up in purple/red: The incorporation of 11‐mercaptoundecanoic acid‐modified gold nanoparticles into loop‐mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion‐template...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2014-04, Vol.10 (8), p.1495-1499
Hauptverfasser: Wong, Jacky K. F., Yip, Shea Ping, Lee, Thomas M. H.
Format: Artikel
Sprache:eng
Schlagworte:
Agglomeration
Amplification
Bacteriophage lambda - genetics
Base Sequence
Byproducts
Color
colorimetric detection
Colorimetry - methods
DNA Primers - genetics
DNA recognition
DNA, Viral - genetics
Fatty Acids - chemistry
Gold
isothermal amplification
Lamps
Magnesium
Metal Nanoparticles - chemistry
Nanoparticles
Nanotechnology
Nucleic Acid Amplification Techniques - methods
Sulfhydryl Compounds - chemistry
Surface Plasmon Resonance
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:LAMP ends up in purple/red: The incorporation of 11‐mercaptoundecanoic acid‐modified gold nanoparticles into loop‐mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion‐templated aggregation, and in the presence of the target, the LAMP reaction by‐product pyrophosphate ion leads to particle deaggregation.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201302348