Color-Discriminating Retinal Configurations of Sensory Rhodopsin I by Photo-Irradiation Solid-State NMR Spectroscopy

SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color‐dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M‐intermediate (attractant) transforms into a P‐intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G‐state. The photoreactions were monitored with the 13C NMR signals of [20‐13C]retnal‐SrSRI using in situ photo‐irradiation solid‐state NMR spectroscopy. The M‐intermediate was trapped at −40 °C by illumination at 520 nm. It was transformed into the P‐intermediate by subsequent illumination at 365 nm. These results reveal that the G‐state could be directly transformed to the P‐intermediate by illumination at 365 nm. Thus, the stationary trapped M‐ and P‐intermediates are responsible for positive and negative phototaxis, respectively. The photocycle of a sensory rhodopsin I (SrSRI) was examined using in situ photo‐irradiation solid‐state NMR spectroscopy. The M‐intermediate was trapped by illumination (520 or 595 nm) and is responsible for the positive phototaxis. The M‐intermediate was transferred to the P‐intermediate (negative phototaxis) by irradiation at 365 nm. These results provide insights into the mechanism of the color‐discriminating functional switch of SrSRI.