Submerged cultivation of mycelium of truffle Tuber magnatum

The possibility of submerged cultivation of mycelium of T. magnatum has been studied in different substrates (glucose, cheese whey and partly deproteinized cheese whey). The yield of mycelium dry substance cultivated in a medium containing 4% of glucose, and 3% of corn steep liquor, was 16.0 g/l (cultivation time - 55 hours). The maximum yield of T. magnatum mycelium dry substance, when cultivated in cheese whey, was achieved after 20 hours of cultivation and amounted to 23.7 g/l. The cultivation parameters were as follows: optimal initial pH value 4.4; specific growth rate mu 0.137 l/h and substrate yield coefficient 0.44 g/g.