Light-sensitive process with immobilized enzyme

Enzyme photoimmobilization was used to create a light-sensitive process. Photoimmobilization of E. coli alkaline phosphatase (EC 3.1.3.1) on paper support was carried out with the help of p-azidobenzaldehyde. The kinetics of the formation of insoluble dye on the carrier with bound enzyme were studie...

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Veröffentlicht in:Enzyme and microbial technology 1989, Vol.11 (8), p.507-510
Hauptverfasser: Kazanskaya, N.F., Manenkova, M.A., Pozharsky, S.B., Berezin, I.V.
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Sprache:eng
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Zusammenfassung:Enzyme photoimmobilization was used to create a light-sensitive process. Photoimmobilization of E. coli alkaline phosphatase (EC 3.1.3.1) on paper support was carried out with the help of p-azidobenzaldehyde. The kinetics of the formation of insoluble dye on the carrier with bound enzyme were studied. The system was shown to be capable of reproducing semitone images with acceptable sensitivity.
ISSN:0141-0229
1879-0909
DOI:10.1016/0141-0229(89)90032-X