Regulation of antibiotic production by iron and oxygen during defined medium fermentations of Streptomyces clavuligerus

When grown in a chemically defined medium, Streptomyces clavuligerus excreted cephamycin C, in addition to other components, throughout most of the growth phase. Ferrous iron and oxygen are required for the biosynthesis of this antibiotic and the concentration of these cofactors was manipulated to m...

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Veröffentlicht in:Applied microbiology and biotechnology 1989-01, Vol.31 (4), p.390-396
Hauptverfasser: ROLLINS, M. J, JENSEN, S. E, WESTLAKE, D. W. S
Format: Artikel
Sprache:eng
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Zusammenfassung:When grown in a chemically defined medium, Streptomyces clavuligerus excreted cephamycin C, in addition to other components, throughout most of the growth phase. Ferrous iron and oxygen are required for the biosynthesis of this antibiotic and the concentration of these cofactors was manipulated to maximize cephamycin C production. The iron content of the chemically defined medium was shown to be sub-optimal for antibiotic production and the addition of 130 mu g/ml ferrous iron almost doubled the cephamycin C levels to 200 mu g/ml. When dissolved oxygen was maintained at saturation levels, only 60-80 mu g/ml cephamycin C was produced, and the intermediate penicillin N accumulated to high levels (50 mu g/ml).
ISSN:0175-7598
1432-0614
DOI:10.1007/bf00257610