Analysis of free sulfhydryl groups and disulfide bonds in Na super(+),K super(+)-ATPase

Analysis of free sulfhydryl groups and disulfide bonds in Na super(+),K super(+)-ATPase

The content of free SH groups and disulfide bonds in the purified pig kidney Na super(+),K super(+)-ATPase was determined by ammetric titration with silver nitrate. In the native enzyme, most of the free SH groups are masked due to their location in the polypeptide chain regions poorly accessible to...

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Veröffentlicht in:FEBS letters 1989-01, Vol.255 (2), p.265-268
Hauptverfasser: Gevondyan, N M, Gevodyan, V S, Gavrilyeva, EE, Modyanov, N N
Format: Artikel
Sprache:eng
Schlagworte:
disulfide bonds
kidney
sulfhydryl groups
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Zusammenfassung:The content of free SH groups and disulfide bonds in the purified pig kidney Na super(+),K super(+)-ATPase was determined by ammetric titration with silver nitrate. In the native enzyme, most of the free SH groups are masked due to their location in the polypeptide chain regions poorly accessible to SH reagents. At least one disulfide bridge is located in the alpha -subunit whereas the beta -subunit contains three disulfide bonds.
ISSN:0014-5793