Agitation-induced aggregation and subvisible particulate formation in model proteins

Agitation-induced aggregation and subvisible particulate formation in model proteins

[Display omitted] The kinetics of agitation-induced subvisible particle formation was investigated for a few model proteins – human serum albumin (HSA), hen egg white lysozyme (HEWL), and a monoclonal antibody (IgG2). Experiments were carried out for the first time under relatively low protein conce...

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Veröffentlicht in:European journal of pharmaceutics and biopharmaceutics 2014-07, Vol.87 (2), p.299-309
Hauptverfasser: Jayaraman, Murali, Buck, Patrick M., Alphonse Ignatius, Arun, King, Kevin R., Wang, Wei
Format: Artikel
Sprache:eng
Schlagworte:
Aggregation
Agitation
Animals
Antibodies, Monoclonal - chemistry
Chickens
Hot Temperature
Humans
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Immunoglobulin G - chemistry
Kinetics
Molecular Weight
Muramidase - chemistry
Particle
Particle Size
Protein
Protein Conformation
Protein Stability
Serum Albumin - chemistry
Serum Albumin, Human
Solubility
Stability
Surface Properties
Unfolding
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Zusammenfassung:[Display omitted] The kinetics of agitation-induced subvisible particle formation was investigated for a few model proteins – human serum albumin (HSA), hen egg white lysozyme (HEWL), and a monoclonal antibody (IgG2). Experiments were carried out for the first time under relatively low protein concentration and low agitation speed to investigate the details of subvisible particle formation at the initial phase of aggregation (
ISSN:0939-6411
1873-3441
DOI:10.1016/j.ejpb.2014.01.004