Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd

This paper to evaluate the in vitro antioxidant and DNA nicking potential of various extracts and fractions of Pterospermum acerifolium (L.) Willd (Family: Stericuliaceae). Antioxidant properties of the extracts and fractions was assayed by DPPH scavenging activity, non-site-specific and site-specific OH radical scavenging activity mediated 2-deoxy- D-ribose degradation, total antioxidant activity and lipid peroxidation assay by using rat liver homogenate. DNA nicking assay was studied by PT257R/T plasmid. Ethyl acetate fractions of leaves, flowers and bark exhibited potent antioxidant property and DNA protective effect compare to all the other extracts and fractions. Total phenolic and flavonoid content determination was also showed ethyl acetate fractions were rich in phenolic and flavonoid contents. The present study indicated that, ethyl acetate fractions of leaves, flowers and bark showed effective antioxidant and DNA protection activity and it could be the initiation for various other pharmacological studies on those fractions.