Effects of grapefruit (Citrus paradisi MACF) (Rutaceae) peel oil against developmental stages of Aedes aegypti (Diptera: Culicidae)

Laboratory bioassay of the essential oil extracted from the grapefruit (Citrus paradisi) peel by steam distillation was carried out against the developmental stages of the yellow fever vector Aedes aegypti to evaluate its toxicity, and ovicidal and larvicidal potency. Volatile oil components isolated and characterized by coupled gas chromatography/mass spectrometry included varying levels of monoterpene aldehydes, alcohols, and esters. Test results of the essential oil showed that egg hatching was completely inhibited at 400 ppm, while further development of 1st to 2nd larval stage was inhibited at 100 ppm. Regression analysis results also indicated that the peel essential oil significantly (p