Obtaining lipases from byproducts of orange juice processing

•Lipases are present in byproducts of orange juice.•High activity in pH from neutral (6–7) to alkaline (8–9) and wide temperature range (20–60°C).•Activities were observed on different pNP-esters (butyrate, laurate and palmitate). The presence of lipases was observed in three byproducts of orange juice processing: peel, core and frit. The enzymes were characterised biochemically over a wide pH range from neutral (6–7) to alkaline (8–9). The optimal temperature for the activity of these byproducts showed wide range at 20°C to 70°C, indicating fairly high thermostability. The activities were monitored on p-NP-butyrate, p-NP-laurate and p-NP-palmitate. For the first time, lipase activity was detected in these residues, reaching 68.5lipaseU/g for the crude extract from fractions called frit.