Rhodanobacter glycinis sp. nov., a yellow-pigmented gammaproteobacterium isolated from the rhizoplane of field-grown soybean

A novel, yellow-pigmented bacterium, designated strain MO64T, was isolated from the rhizoplane of field-grown soybean, collected from an experimental plot at Coimbatore, India. Cells were Gram-reaction-negative, motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain MO64T belonged to the genus Rhodanobacter. Strain MO64T was related most closely to Rhodanobacter ginsengisoli GR17-7T (98.0 % 16S rRNA gene sequence similarity), Rhodanobacter spathiphylli B39T (97.9 %), Rhodanobacter panaciterrae LnR5-47T (97.7 %), Rhodanobacter terrae GP18-1T (97.6 %), Rhodanobacter soli DCY45T (97.3 %) and Rhodanobacter caeni MJ01T (97.2 %); levels of similarity to the type strains of all other recognized species of the genus Rhodanobacter were less than 97.0 %. Chemotaxonomic data (Q-8 as the predominant ubiquinone, and iso-C16 : 0, iso-C15 : 0, C17 : 0 cyclo, iso-C17 : 1ω9c, iso-C17 : 0 and iso-C11 : 0 as the major fatty acids) also supported the affiliation of strain MO64T with the genus Rhodanobacter. The G+C content of the genomic DNA was 64 mol%. The results of DNA–DNA hybridization and phenotypic analysis showed that strain MO64T can be distinguished from all known species of the genus Rhodanobacter and therefore represents a novel species of the genus, for which the name Rhodanobacter glycinis sp. nov. is proposed. The type strain is MO64T ( = ICMP 17626T = NBRC 105007T).