Hydrolysis of a phospholipid in an inert lipid matrix by phospholipase A sub(2): A super(13)C NMR study

A new approach to study phospholipase A sub(2) mediated hydrolysis of phospholipid vesicles, using super(13)C NMR spectroscopy, is described. ( super(13)C)Carbonyl-enriched dipalmitoyl-phosphatidylcholine (DPPC) incorporated into nonhydrolyzable either-linked phospholipid bilayers was hydrolyzed by...

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Veröffentlicht in:Biochemistry (Easton) 1989-01, Vol.28 (16), p.6667-6672
Hauptverfasser: Bhamidipati, S P, Hamilton, JA
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Sprache:eng
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Zusammenfassung:A new approach to study phospholipase A sub(2) mediated hydrolysis of phospholipid vesicles, using super(13)C NMR spectroscopy, is described. ( super(13)C)Carbonyl-enriched dipalmitoyl-phosphatidylcholine (DPPC) incorporated into nonhydrolyzable either-linked phospholipid bilayers was hydrolyzed by phospholipase A sub(2) (Crotalus adamanteus)).
ISSN:0006-2960