Evaluation of reduction kinetics of 2,2-diphenyl-1-picrylhydrazyl radical by flavonoid glycoside Rutin in mixed solvent based micellar media

We report effect of micellar aggregates on DPPH reduction kinetics by antiradical Rutin. •We report effect of micellar media in mixed solvent on DPPH reduction kinetics.•DPPH reduction was faster in post micellar range except in non-ionic surfactant.•Binary surfactant mixtures were highly efficient compared to single systems.•Partitioning and interaction mode of Rutin had great effect on DPPH kinetics. The work evaluates the reduction kinetics of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by Rutin in different mixed solvent based micellar media comprising of various cationic, anionic, zwitterionic, nonionic and their binary mixtures using spectrophotometric, fluorimetric and tensiometric methods. The analysis of kinetic measurements in micellar systems was done using models proposed by Goupy et al. and Sendra et al. applicable to initial and overall DPPH reduction kinetics respectively. The rate constants observed in the methanol–water media containing micelles (except in cationic micellar medium) were significantly faster than those without micelles suggesting the use of such media as efficient systems for evaluation of DPPH activity in comparison with the pure organic solvents having environmental concern. Rate of DPPH reduction was dependent on nature as well as concentration of amphiphile used. The results were explained on the basis of compartmentalization and mode of interaction of Rutin with micellar nanostructures.