Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene
Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2014-06, Vol.79 (2), p.240-241 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Caldas, Paulo Cesar de Souza Campos, Carlos Eduardo Dias dos Reis, Lusiano Motta Ferreira, Nicole Victor de Carvalho, Luciana Distásio da Silva, Mariza Villas Boas Medeiros, Reginalda Ferreira de Melo Montes, Fátima Cristina Onofre Fandinho Ramos, Jesus Pais |
| description | Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile. |
| doi_str_mv | 10.1016/j.diagmicrobio.2014.01.028 |
| format | Article |
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The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2014.01.028</identifier><identifier>PMID: 24655570</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacterial Proteins - genetics ; Brazil ; Chaperonin 60 - genetics ; Cluster Analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Humans ; Infectious Disease ; Internal Medicine ; Molecular Sequence Data ; Molecular Typing ; Mycobacterium avium Complex - classification ; Mycobacterium avium Complex - genetics ; Mycobacterium avium Complex - isolation & purification ; Mycobacterium avium-intracellulare Infection - microbiology ; Mycobacterium intracellulare ; Nontuberculous mycobacteria ; Phylogeny ; Pneumonia - microbiology ; Polymerase Chain Reaction ; PRA-hsp65 ; Restriction Mapping ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sputum - microbiology</subject><ispartof>Diagnostic microbiology and infectious disease, 2014-06, Vol.79 (2), p.240-241</ispartof><rights>Elsevier Inc.</rights><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c378t-4bae1d72bece293858300dc72aea89529a8a887755409493ac71518b2fd9f2e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889314000613$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24655570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caldas, Paulo Cesar de Souza</creatorcontrib><creatorcontrib>Campos, Carlos Eduardo Dias</creatorcontrib><creatorcontrib>dos Reis, Lusiano Motta</creatorcontrib><creatorcontrib>Ferreira, Nicole Victor</creatorcontrib><creatorcontrib>de Carvalho, Luciana Distásio</creatorcontrib><creatorcontrib>da Silva, Mariza Villas Boas</creatorcontrib><creatorcontrib>Medeiros, Reginalda Ferreira de Melo</creatorcontrib><creatorcontrib>Montes, Fátima Cristina Onofre Fandinho</creatorcontrib><creatorcontrib>Ramos, Jesus Pais</creatorcontrib><title>Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile.</description><subject>Bacterial Proteins - genetics</subject><subject>Brazil</subject><subject>Chaperonin 60 - genetics</subject><subject>Cluster Analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Internal Medicine</subject><subject>Molecular Sequence Data</subject><subject>Molecular Typing</subject><subject>Mycobacterium avium Complex - classification</subject><subject>Mycobacterium avium Complex - genetics</subject><subject>Mycobacterium avium Complex - isolation & purification</subject><subject>Mycobacterium avium-intracellulare Infection - microbiology</subject><subject>Mycobacterium intracellulare</subject><subject>Nontuberculous mycobacteria</subject><subject>Phylogeny</subject><subject>Pneumonia - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>PRA-hsp65</subject><subject>Restriction Mapping</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sputum - microbiology</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2PljAQhxujcV9X_wVDPHkBp4XS1oOJedevZI3Gj3NTyrAWgWILGvzrLfuuxnjy1EOf-c3MM4Q8olBQoPWTvmiduRqdDb5xvmBAqwJoAUzeIgcqhcoBBNwmBxAly6VU5Rm5F2MPQJmq4C45Y1XNORdwIP0FRhvcvDg_Zb7LTDbhj-ztZn1j7ILBrWPmpiUYi8OwDiZgNpslfVzT748fsoBxCc5eB-D0cxsxM5MZtujijnyJc82zK5zwPrnTmSHig5v3nHx--eLT8XV--e7Vm-Pzy9yWQi551RikrWANWmSqlFyWAK0VzKCRijNlpJFSCM4rUJUqjRWUU9mwrlUdQyjPyeNT7hz8tzVNp0cX9_HNhH6NmnJWJxGK0YQ-PaFJZYwBOz0HN5qwaQp6d617_bdrvbvWQHVynYof3vRZmxHbP6W_5Sbg4gRg2va7w6CjdThZbF1Au-jWu__r8-yfGDu4yVkzfMUNY-_XkHynvXRkGvTH_er70WkFADUty1_hAqyD</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Caldas, Paulo Cesar de Souza</creator><creator>Campos, Carlos Eduardo Dias</creator><creator>dos Reis, Lusiano Motta</creator><creator>Ferreira, Nicole Victor</creator><creator>de Carvalho, Luciana Distásio</creator><creator>da Silva, Mariza Villas Boas</creator><creator>Medeiros, Reginalda Ferreira de Melo</creator><creator>Montes, Fátima Cristina Onofre Fandinho</creator><creator>Ramos, Jesus Pais</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene</title><author>Caldas, Paulo Cesar de Souza ; Campos, Carlos Eduardo Dias ; dos Reis, Lusiano Motta ; Ferreira, Nicole Victor ; de Carvalho, Luciana Distásio ; da Silva, Mariza Villas Boas ; Medeiros, Reginalda Ferreira de Melo ; Montes, Fátima Cristina Onofre Fandinho ; Ramos, Jesus Pais</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-4bae1d72bece293858300dc72aea89529a8a887755409493ac71518b2fd9f2e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Brazil</topic><topic>Chaperonin 60 - genetics</topic><topic>Cluster Analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Internal Medicine</topic><topic>Molecular Sequence Data</topic><topic>Molecular Typing</topic><topic>Mycobacterium avium Complex - classification</topic><topic>Mycobacterium avium Complex - genetics</topic><topic>Mycobacterium avium Complex - isolation & purification</topic><topic>Mycobacterium avium-intracellulare Infection - microbiology</topic><topic>Mycobacterium intracellulare</topic><topic>Nontuberculous mycobacteria</topic><topic>Phylogeny</topic><topic>Pneumonia - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>PRA-hsp65</topic><topic>Restriction Mapping</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sputum - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caldas, Paulo Cesar de Souza</creatorcontrib><creatorcontrib>Campos, Carlos Eduardo Dias</creatorcontrib><creatorcontrib>dos Reis, Lusiano Motta</creatorcontrib><creatorcontrib>Ferreira, Nicole Victor</creatorcontrib><creatorcontrib>de Carvalho, Luciana Distásio</creatorcontrib><creatorcontrib>da Silva, Mariza Villas Boas</creatorcontrib><creatorcontrib>Medeiros, Reginalda Ferreira de Melo</creatorcontrib><creatorcontrib>Montes, Fátima Cristina Onofre Fandinho</creatorcontrib><creatorcontrib>Ramos, Jesus Pais</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caldas, Paulo Cesar de Souza</au><au>Campos, Carlos Eduardo Dias</au><au>dos Reis, Lusiano Motta</au><au>Ferreira, Nicole Victor</au><au>de Carvalho, Luciana Distásio</au><au>da Silva, Mariza Villas Boas</au><au>Medeiros, Reginalda Ferreira de Melo</au><au>Montes, Fátima Cristina Onofre Fandinho</au><au>Ramos, Jesus Pais</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>79</volume><issue>2</issue><spage>240</spage><epage>241</epage><pages>240-241</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24655570</pmid><doi>10.1016/j.diagmicrobio.2014.01.028</doi><tpages>2</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Proteins - genetics Brazil Chaperonin 60 - genetics Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Humans Infectious Disease Internal Medicine Molecular Sequence Data Molecular Typing Mycobacterium avium Complex - classification Mycobacterium avium Complex - genetics Mycobacterium avium Complex - isolation & purification Mycobacterium avium-intracellulare Infection - microbiology Mycobacterium intracellulare Nontuberculous mycobacteria Phylogeny Pneumonia - microbiology Polymerase Chain Reaction PRA-hsp65 Restriction Mapping RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sputum - microbiology |
| title | Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene |
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