Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene

Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2014-06, Vol.79 (2), p.240-241
Hauptverfasser:	Caldas, Paulo Cesar de Souza, Campos, Carlos Eduardo Dias, dos Reis, Lusiano Motta, Ferreira, Nicole Victor, de Carvalho, Luciana Distásio, da Silva, Mariza Villas Boas, Medeiros, Reginalda Ferreira de Melo, Montes, Fátima Cristina Onofre Fandinho, Ramos, Jesus Pais
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Brazil Chaperonin 60 - genetics Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Humans Infectious Disease Internal Medicine Molecular Sequence Data Molecular Typing Mycobacterium avium Complex - classification Mycobacterium avium Complex - genetics Mycobacterium avium Complex - isolation & purification Mycobacterium avium-intracellulare Infection - microbiology Mycobacterium intracellulare Nontuberculous mycobacteria Phylogeny Pneumonia - microbiology Polymerase Chain Reaction PRA-hsp65 Restriction Mapping RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sputum - microbiology
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Zusammenfassung:	Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2014.01.028