A syncytia assay for human immunodeficiency virus type I (HIV-1) envelope protein and its use in studying HIV-1 mutations

A syncytia assay for human immunodeficiency virus type I (HIV-1) envelope protein and its use in studying HIV-1 mutations

The authors describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type (HIV-1). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 ce...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1989-01, Vol.170 (2), p.566-570
Hauptverfasser: Felser, J M, Klimkait, T, Silver, J
Format: Artikel
Sprache:eng
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Zusammenfassung:The authors describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type (HIV-1). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 cells. This plasmid was used to study effects of mutations in the gp120 and gp41 portions of the envelope gene of HIV-1.
ISSN:0042-6822