Enzymatic amplification for spectrophotometric and electrochemical assays of NAD super(+) and NADH

Enzymatic amplification for spectrophotometric and electrochemical assays of NAD super(+) and NADH

Five different procedures are presented for the enzymatic assay of the sum of NAD super(+) and NADH concentrations. They are based on the principle of amplification by cycling. The reactions involve oxidation of the formate ion, ethanol, glucose, or carnitine catalyzed by the corresponding dehydroge...

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Veröffentlicht in:Analytical biochemistry 1989-01, Vol.179 (2), p.382-388
Hauptverfasser: Bergel, A, Souppe, J, Comtat, M
Format: Artikel
Sprache:eng
Schlagworte:
NADH
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Zusammenfassung:Five different procedures are presented for the enzymatic assay of the sum of NAD super(+) and NADH concentrations. They are based on the principle of amplification by cycling. The reactions involve oxidation of the formate ion, ethanol, glucose, or carnitine catalyzed by the corresponding dehydrogenases. The detection reactions are based on the 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)/INT-formazan and ferricyanide/ferrocyanide couples and use a diaphorase.
ISSN:0003-2697