Broad heparin‐binding haemagglutinin‐specific cytokine and chemokine response in infants following Mycobacterium bovis BCG vaccination

Heparin‐binding haemagglutinin (HBHA)‐specific immune responses have been linked to protection against tuberculosis (TB). We investigated the hypothesis that BCG vaccination of human infants primes an HBHA‐specific response, using multiplex to measure secreted cytokines and chemokines following HBHA...

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Veröffentlicht in:European journal of immunology 2012-09, Vol.42 (9), p.2511-2522
Hauptverfasser: Smith, Steven G., Lecher, Sophie, Blitz, Rose, Locht, Camille, Dockrell, Hazel M.
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Sprache:eng
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Zusammenfassung:Heparin‐binding haemagglutinin (HBHA)‐specific immune responses have been linked to protection against tuberculosis (TB). We investigated the hypothesis that BCG vaccination of human infants primes an HBHA‐specific response, using multiplex to measure secreted cytokines and chemokines following HBHA and Mycobacterium tuberculosis purified protein derivative (PPD) stimulation of diluted whole blood samples from BCG‐vaccinated or ‐unvaccinated infants. Of 42 analytes measured, 24 and 32 significant, BCG‐associated increases were detected in response to HBHA and PPD, respectively. Both response profiles included Th‐1, Th‐2, Th‐17 and inflammatory cytokines and chemokines (e.g. IFN‐γ, TNF‐α, IL‐5, IL‐10, IL‐13, IL‐17, MIP‐1α and MIP‐1β). We also found that six of the seven responses most closely correlated with IFN‐γ were common to both HBHA and PPD. Notably, all HBHA‐specific secretion of cytokines and chemokines from infant samples was dependant on previous BCG vaccination. Also, long‐term persistence of HBHA‐specific responses was found in adolescents with evidence of infant BCG vaccination. This study demonstrates for the first time BCG priming of an HBHA‐specific immune response in infants that is characterised by a broad cytokine and chemokine signature. It also suggests a number of BCG vaccination associated, HBHA‐induced responses that should be useful for future studies of biomarkers of protection against TB.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201142297