A yeast tRNA super(Arg) gene can act as promoter for a 5' flank deficient, non-transcribable tRNA super(SUP6) gene to produce biologically active suppressor tRNA

In S. cerevisiae most tRNA genes are located and expressed as single entities. The tDNA super(Arg)-tDNA super(Asp) pair, however, is transcribed into a dimeric precursor before being processed into two mature tRNA species. The second gene of this pair, tDNA super(Asp), is totally dependent on the first gene, tDNA super(Arg), and its promoter components, for homologous in vitro transcription. tDNA super(Arg) with its 5' flanking region can act as an external promoter for other RNA polymerase III-read genes that are by themselves inactive due to impaired promoter/modulator regions.