A new method for yeast phagocytosis analysis by flow cytometry

Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence with...

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Veröffentlicht in:	Journal of microbiological methods 2014-06, Vol.101, p.56-62
Hauptverfasser:	Carneiro, Catarina, Vaz, Catarina, Carvalho-Pereira, Joana, Pais, Célia, Sampaio, Paula
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Candida - cytology Candida - isolation & purification Candida spp Cell Line Flow cytometry Flow Cytometry - methods Macrophages - physiology Mice Organic Chemicals PAMPs Phagocytosis Phagocytosis - physiology Propidium Propidium iodide Sytox Green
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Zusammenfassung:	Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. •New simple and fast flow cytometry method for yeast species phagocytosis•Different Candida species present different interactions with macrophages.•Quenching effect of PI over Sytox Green fluorescence of the labeled yeast cells
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2014.03.013