Sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in Fusarium oxysporum 26-1

Two protease-like proteins, KrtA and KrtC, were identified in Fusarium oxysporum 26-1. Genes coding these proteins, krtA and krtC, were isolated and characterized. Recombinant KrtA (rKrtA) and KrtC (rKrtC) were successfully expressed in Aspergillus oryzae and secreted. The combination of rKrtA and r...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of bioscience and bioengineering 2014-06, Vol.117 (6), p.711-714
Hauptverfasser:	Chaya, Etsushi, Suzuki, Tohru, Karita, Shuichi, Hanya, Akira, Yoshino-Yasuda, Shoko, Kitamoto, Noriyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Aspergillus oryzae Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - genetics Fusarium - enzymology Fusarium - genetics Fusarium oxysporum Heterologous expression Molecular Sequence Data Peptide Hydrolases - chemistry Peptide Hydrolases - genetics Protease Sequence Analysis, Protein Sheep Wool - chemistry Wool - ultrastructure Wool cuticle
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Two protease-like proteins, KrtA and KrtC, were identified in Fusarium oxysporum 26-1. Genes coding these proteins, krtA and krtC, were isolated and characterized. Recombinant KrtA (rKrtA) and KrtC (rKrtC) were successfully expressed in Aspergillus oryzae and secreted. The combination of rKrtA and rKrtC completely removed the cuticle of wool fibers.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2013.11.012