Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase

Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoi...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2014-06, Vol.117 (6), p.676-679
Hauptverfasser:	Kojima, Yusuke, Wakita, Jun, Inayoshi, Yujin, Suzuki, Ryosuke, Yamada, Yuki, Kaneoka, Hidenori, Nishijima, Ken-ichi, Iijima, Shinji
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified - genetics Avian Proteins - biosynthesis Avian Proteins - genetics Biological and medical sciences Biotechnology Chick Embryo Chickens - genetics Egg Proteins - metabolism Erythropoietin Erythropoietin - genetics Erythropoietin - metabolism Fundamental and applied biological sciences. Psychology Galactose Galactose - metabolism Galactosyltransferase Galactosyltransferases - biosynthesis Galactosyltransferases - genetics Glycosylation Humans N-Glycan Plasmids Protein Processing, Post-Translational Transgenic chicken Vesiculovirus - genetics
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Beschreibung
Zusammenfassung:	Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2013.11.010