Generation of Recombinant Extracellular Fragment of Vascular Endothelial Growth Factor Receptor 2 and Specific Monoclonal Antibodies to This Receptor

cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET_32a. Western blotting showed immunochemical identity of recombinant VEGFR2 I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2 I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.