Soluble, nitrate/nitrite-inducible cytochrome P-450 of the fungus, Fusarium oxysporum

Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositio...

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Veröffentlicht in:	FEBS letters 1989-02, Vol.244 (1), p.11-14
Hauptverfasser:	Shoun, Hirofumi, Suyama, Wakako, Yasui, Tsuneo
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbon Monoxide - metabolism Cytochrome P-450 Cytochrome P-450 Enzyme System - biosynthesis cytochrome P450 Enzyme Induction - drug effects Fatty acid hydroxylase Fusarium - enzymology Fusarium oxysporum microsomes Microsomes - enzymology Nitrate/nitrite-mediated induction Nitrates - pharmacology Nitrites - pharmacology P-450 Sodium Nitrite - pharmacology Solubility Spectrophotometry
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Beschreibung
Zusammenfassung:	Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO complex of the soluble P-450 exhibited an absorption spectrum that is different from that of the microsomal counterpart. These results indicate that the soluble P-450 is distinct from the microsomal species and suggest a novel function for the former P-450.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(89)81151-2