A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method usi...

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Veröffentlicht in:Analytical biochemistry 2014-05, Vol.452, p.13-15
Hauptverfasser: Zheng, Jianyong, Fu, Xianfeng, Ying, Xiangxian, Zhang, Yinjun, Wang, Zhao
Format: Artikel
Sprache:eng
Schlagworte:
Butanols - metabolism
Colorimetry - methods
Derivatization
Enzyme Assays - methods
High-throughput screening
High-Throughput Screening Assays
Lipase
Lipase - metabolism
MBTH
Synthetic activity
Vinyl Compounds - metabolism
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Beschreibung
Zusammenfassung:A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2014.02.001