New food for an old mouth: New enzyme for an ancient archaea

•Contrary to literature, the presence of GSH and GSTs were shown in archaea.•Amounts of GSH were measured in Haloarcula hispanica.•H. hispanica GSTs specific activities were determined as 19.68nmolmin−1mg−1 protein.•Vmax and Km values for H. hispanica GST activity toward CDNB and GSH were calculated.•It is the first report on this area. As a multifunctional group of enzymes, glutathione S-transferases (GSTs) are capable of inactivation, degradation or excretion of wide range of compounds catalytically or non-catalytically. However, to date, no study has been addresses the presence of GSTs in archaea based on their enzymatic functions. In this study, beside glutathione (GSH) amount measurement, the determination of GST activity in halophilic archaeon called Haloarcula hispanica ATCC 33960 were aimed. According to the results, specific activity was determined as 19.68nmolmin−1mg−1 protein and GSH content were found to be as 194μgg−1Km and Vmax values for CDNB and GSH calculated from Lineweaver-Burk plot were 0.46mM and 27.93nmolmin−1mg−1, 0.13mM and 22.03nmolmin−1mg−1, respectively. Hanes-Woolf and Eadie-Hofstee plots for CDNB and GSH were also found to be in co-relation with the results obtained from Lineweaver-Burk plot. To the best of our knowledge, GST enzymes have not been identified in archaea yet, at least based on their catalytic activities. Therefore, it is the first report on this area.