Identification of biomarkers to detect residual pertussis toxin using microarray analysis of dendritic cells

Identification of biomarkers to detect residual pertussis toxin using microarray analysis of dendritic cells

Highlights • Microarray was used to identify markers to detect pertussis toxin in six human cell types. • Immature monocyte-derived dendritic cells (iMoDCs) responded to pertussis toxin. • PTx-responsive gene profile in iMoDCs: IFNG , IL2 , XCL1 , CD69 , CSF2 and CXCL10. • PTx-induced IL-2 and IFN-γ...

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Veröffentlicht in:Vaccine 2013-10, Vol.31 (45), p.5223-5231
Hauptverfasser: Vaessen, S.F.C, Verkoeijen, S, Vandebriel, R.J, Bruysters, M.W.P, Pennings, J.L.A, Bos, R, Krul, C.A.M, Akkermans, A.M
Format: Artikel
Sprache:eng
Schlagworte:
Allergy and Immunology
Aluminum
Applied microbiology
Biological and medical sciences
Biomarkers
Biomarkers, Pharmacological - analysis
Bordetella pertussis
Cell culture
Cells, Cultured
chemokine CXCL10
Chemokines
Dendritic cells
Dendritic Cells - drug effects
endothelial cells
epithelial cells
Escherichia coli
fibroblasts
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling
gene expression regulation
genes
Glycerol
human cell lines
Humans
hybrids
IL-2
Immunization
interferon-gamma
interleukin-2
Ligands
lipopolysaccharides
Microarray
Microarray Analysis
microarray technology
Microbiology
myocytes
Pertussis toxin
Pertussis Toxin - analysis
Pertussis Toxin - toxicity
Pertussis Vaccine - standards
proteins
Public health
Pulmonary arteries
pulmonary artery
Safety
Smooth muscle
Statistical analysis
Technology, Pharmaceutical - methods
Toxins
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Beschreibung
Zusammenfassung:Highlights • Microarray was used to identify markers to detect pertussis toxin in six human cell types. • Immature monocyte-derived dendritic cells (iMoDCs) responded to pertussis toxin. • PTx-responsive gene profile in iMoDCs: IFNG , IL2 , XCL1 , CD69 , CSF2 and CXCL10. • PTx-induced IL-2 and IFN-γ responses in iMoDCs were sensitive and specific compared to pertussis toxoid, LPS and LOS. • IL2 is a promising candidate to be used as a biomarker for the detection of residual PTx.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2013.08.082