Combined high-performance liquid chromatographic-radioimmunoassay method for the analysis of endorphins, enkephalins and other neurotransmitter peptides

Assays for beta -endorphin (BE) and its precursors such as beta -lipotropin (LPH) in cerebrospinal fluid (CSF), plasma and some tissues have been difficult because of their low concentrations in limited sample volumes, the non-specificity of most antisera. This study reports a high-performance liqui...

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Veröffentlicht in:	Journal of Chromatography A 1987, Vol.423, p.93-104
1. Verfasser:	VENN, R. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry and metabolism Biological and medical sciences Central nervous system cerebrospinal fluid Fundamental and applied biological sciences. Psychology man opioid peptides Vertebrates: nervous system and sense organs
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Assays for beta -endorphin (BE) and its precursors such as beta -lipotropin (LPH) in cerebrospinal fluid (CSF), plasma and some tissues have been difficult because of their low concentrations in limited sample volumes, the non-specificity of most antisera. This study reports a high-performance liquid chromatographic (HPLC) separation method in which BE and LPH are well separated from each other and which also separates other neuropeptides of interest. The method uses volatile solvents which do not interfere with radioimmunoassay (RIA).
ISSN:	0021-9673